日本金属学会誌

J. Japan Inst. Met. Mater, Vol. 79, No. 1 (2015),
pp. 1-8

Direct and Rapid Quantification of Calcium Phosphate Precipitate on Titanium by X-Ray Fluorescence Analysis Using Fundamental Parameter Method

Kozue Satoh1, Masashi Komata2, Manabu Mizuhira3, Kazuaki Wagatsuma4 and Naofumi Ohtsu1

1Instrumental Analysis Center, Kitami Institute of Technology, Kitami 090-8507
2Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Kitami 090-8507
3Bruker AXS K. K., Yokohama 221-0022
4Institute for Materials Research, Tohoku University, Sendai 980-8577

Abstract:

In the present study, we propose a characterization technique to determine the amount of calcium phosphate (CP) precipitate formed on a titanium substrate. The quantitative analysis of the CP precipitate on a metallic substrate is significant for researchers of metallic biomaterials because CP that spontaneously precipitates in a simulated-body fluid gives information on the bioactivity of the metallic biomaterials. We focused on X-ray fluorescence (XRF) analysis and adopted the thin-film fundamental parameter method (thin-film FP method) because it allows direct (non-pretreatment) and rapid quantitative analysis without any reference materials. We show that XRF analysis using the thin-film FP method can be adequately applied to the quantitative analysis of the CP precipitate in the simulated-body fluid immersion test. We also show that the density of the CP-precipitate layer can be estimated by combining the XRF results with images of cross-sectional scanning electron microscopy (SEM).
Consequently, XRF analysis combined with the thin-film FP method can provide a convenient means to evaluate the CP precipitate on a titanium substrate, which improves the accuracy and accessibility of the simulated-body fluid immersion test. [doi:10.2320/jinstmet.J2014026]


(Received 2014/4/11)

Keywords:

X-ray fluorescence analysis, fundamental parameter method, thin film, calcium phosphate, titanium, metallic biomaterial, bioactivity


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