Materials Transactions Online

Materials Transactions, Vol.59 No.01 (2018) pp.72-81
© 2017 The Mining and Materials Processing Institute of Japan

Factors Affecting Sand Solidification Using MICP with Pararhodobacter sp.

G.G.N.N. Amarakoon1 and Satoru Kawasaki2

1Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan
2Faculty of Engineering, Hokkaido University, Sapporo 060-8628, Japan

Biomineralization is an environmentally friendly technology for improving soil-engineering properties. One of the most common biomineralization processes is microbially induced calcite precipitation (MICP). In this study, sand solidification tests were conducted using Pararhodobacter sp., which is a local ureolytic bacteria obtained from the sand near beach rock in Okinawa, Japan. The goal of this study was to solidify a specimen having an estimated unconfined compressive strength (UCS) of more than several MPa to improve soil properties and investigate the influence of various factors on the engineering properties of treated soil catalyzed by ureolytic bacteria (curing temperature, injection interval of cementation solution, Ca2+ concentration, curing time, bacterial population, re-injection of bacteria and particle size of sand). Model test specimens were cemented up to an estimated UCS of 10 MPa after 14 days under the following conditions: a curing temperature of 30℃, an injection interval of 1 day, and a Ca2+ concentrations in cementation solution of 0.5 M. Multiple regression analysis showed that the relevant conditions for estimating UCS were test period, D (days), and Ca2+ concentration of the cementation solution, Cca (M). The formula for predicting the estimated UCS (qeu (MPa)) was qeu = 13.99 Cca + 0.37 D − 0.09. Overall, the results of this study will contribute to the application of a new technique to sand improvement and bio-stimulation.


(Received 2017/03/07; Accepted 2017/10/25; Published 2017/12/25)

Keywords: biomineralization, microbially induced calcite precipitation (MICP), urease, unconfined compressive strength

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  1. J.T. DeJong, M.B. Fritzges and K. Nusslein: J. Geotech. Geoenviron. Eng. 132 (2006) 1381-1392.
  2. V.S. Whiffin, L.A. van Paassen and M.P. Harkes: Journal of Geomicrobiology 24 (2007) 417-423.
  3. L.A. van Paassen, C.M. Daza, M. Staal, D.Y. Sorokin, W. van der Zonb and M.C. van Loosdrecht: Ecol. Eng. 36 (2010) 168-175.
  4. B.M. Mortensen, M.J. Haber, J.T. DeJong, L.F. Caslake and D.C. Nelson: J. Appl. Microbiol. 111 (2011) 338-349.
  5. J.K. Mitchell and C.J. Santamarina: J. Geotech. Geoenviron. Eng. 131 (2005) 1222-1233.
  6. V. Rebata-Landa: Ph.D. Thesis, Georgia Institute of Technology, Atlanta, GA (2007).
  7. Y. Fujita, E.G. Ferris, R.D. Lawson, F.S. Colwell and R.W. Smith: Journal of Geomicrobiol. 17 (2000) 305-318.
  8. B.U. Foesel, H.L. Darke and A. Schramm: Journal of Systematic and applied microbiology 34 (2011) 498-502.
  9. A.Al. Qabany, K. Soga and C. Santamarina: J. Geotech. Geoenviron. Eng. 138 (2012) 992-1001.
  10. L.A. van Paassen, M.P. Harkes, G.A. van Zwieten, W.H. van der Zon, W.R.L. van der Star and M.C.M. van Loosdrecht: 17th International Conference on Soil Mechanics and Geotechnical Engineering, (2009) pp. 2328-2333.
  11. L. Cheng, R. Cord-Ruwisch and M.A. Shahin: Can. Geotech. J. 50 (2013) 81-90.
  12. T. Danjo: Doctoral Thesis, Hokkaido University, Japan (2015).
  13. T. Danjo and S. Kawasaki: Mater. Trans. 57 (2016) 428-437.
  14. S. Al-Thawadi and R. Cord-Ruwisch: J. Adv. Sci. Eng. Res. 2 (2012) 13-26.
  15. G.G.N.N. Amarakoon: Doctoral Thesis, Hokkaido University, Japan (2016).


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