Materials Transactions Online

Materials Transactions, Vol.57 No.12 (2016) pp.2020-2025
© 2016 The Japan Institute of Metals and Materials

Cytocompatibility of Ti-6Al-7Nb through High-Pressure Torsion Processing

Peng Chen1, Maki Ashida1, Hisashi Doi1, Yusuke Tsutsumi1, 2, Zenji Horita3, 4 and Takao Hanawa1

1Department of Metallic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Japan
2Graduate School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan
3Department of Materials Science and Engineering, Kyushu University, Fukuoka 819-0395, Japan
4WPI, International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, Fukuoka 819-0395, Japan

In this study, we evaluated the cytocompatibility of high-pressure torsion (HPT)-processed Ti-6Al-7Nb alloys. Grain size of titanium (Ti) alloys decreased from 5 µm (without HPT processing, HPT-0) to ~100 nm (at 2 GPa, HPT-2) or ~70 nm (at 6 GPa, HPT-6) through HPT processing. Cell adhesion, proliferation, and differentiation were evaluated with mouse preosteoblast (MC3T3-E1). A locomotion trend was presented by cells cultured on HPT-2 to compare with other specimens, while, an immobilization trend was presented by cells cultured on HPT-6 to compare with other specimens. Akp2 (alkaline phosphatase 2) expression was higher in cells cultured on HPT-2 than that on HPT-0 and HPT-6, indicating enhanced osteogenic differentiation. Our results demonstrate that HPT-processed Ti-6Al-7Nb alloys offer good cytocompatibility.

[doi:10.2320/matertrans.MI201515]

(Received 2016/05/30; Accepted 2016/10/05; Published 2016/11/25)

Keywords: titanium alloys, high-pressure torsion, adhesion, osteogenic differentiation

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