Materials Transactions Online

Materials Transactions, Vol.53 No.05 (2012) pp.980-984
© 2012 The Japan Institute of Metals

Analysis of Biological Apatite Crystal Orientation in Anterior Cortical Bone of Human Mandible Using Microbeam X-ray Diffractometry

Hidetaka Furuya1, 2, Satoru Matsunaga1, 2, Yuichi Tamatsu3, Takayoshi Nakano4, Masao Yoshinari2, Yoshinobu Ide1 and Shinichi Abe1

1Department of Anatomy, Tokyo Dental College, Chiba 261-8502, Japan
2Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, Chiba 261-8502, Japan
3Department of Neurology Gross Anatomy Section, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan
4Divison of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan

The human jaw is a unique bone that facilitates mastication. The structural properties of the jaw are determined by mechanical stresses transmitted via the teeth. However, it is very difficult to evaluate the impact of these mechanical stresses on bone. In recent years, it has become clear that the orientation of biological apatite (BAp) crystals is closely related to local stress, and is thought to respond more acutely to local stress than bone mineral density (BMD). Few studies have been conducted on BAp crystal alignment in response to mechanical stress in the human jaw, which has a complex masticatory function. The purpose of this study was to quantitatively evaluate BMD and BAp crystal orientation using micro-computed tomography (micro-CT) and microbeam X-ray diffractometry in the anterior cortical bone of human mandible. The intensity and direction of mechanical stresses in both the alveolar area and mandibular base were compared.

The mandibular central incisor region in Japanese bone samples was designated as the region of interest and BMD and BAp crystal orientation in the alveolar area and mandibular base measured. Bone samples were imaged by micro-CT and the data obtained converted into BMD values. BAp crystal orientation was determined by both reflection- and transmission-based microbeam X-ray diffractometry. The diffraction intensity ratio was calculated using X-ray diffraction peaks of (002) and (310).

The results showed no difference in BMD values among regions. BAp crystals were oriented predominantly in the mesiodistal direction in the mandibular base and along the direction of masticatory force in the alveolar area. These findings suggest that the mandibular base exhibits long bone-like characteristics, with the mandibular condyle acting as the head of the bone, while in the alveolar area alignment takes place in the direction of masticatory force resulting from mechanical stress exerted via the teeth. Qualitative evaluation revealed clear differences between the mandibular base and alveolar area, suggesting that BAp crystal orientation offers a more precise indicator of bone quality than BMD.

(Received 2011/10/25; Accepted 2012/02/08; Published 2012/04/25)

Keywords: human mandible, biological apatite crystallite, bone quality, microbeam X-ray diffraction, transmission method

PDF(Free)PDF (Free) Table of ContentsTable of Contents

REFERENCES

  1. J. Wolff: Virchows Arch. Pathol. Anat. Physiol. Klin. Med. 50 (1870) 389-450.
  2. H. M. Frost: Anat. Rec. 219 (1987) 1-9.
  3. Y. Ide and H. Agematsu: Jpn. J. Oral Biol. 39 (1997) 79-90.
  4. K. Nakajima, Y. Onoda, M. Okada, S. Abe and Y. Ide: Bull. Tokyo Dent. Coll. 39 (1998) 57-65.
  5. I. Ichim, J. A. Kieser and M. V. Swain: Arch. Oral Biol. 52 (2007) 465-473.
  6. T. Nakano, K. Kaibara, Y. Tabata, N. Nagata, S. Enomoto, E. Marukawa and Y. Umakoshi: Bone 31 (2002) 479-487.
  7. M. Ito, T. Nakamura, T. Matsumoto, K. Tsurusaki and K. Hayashi: Bone 23 (1998) 163-169.
  8. E. Vega, G. Ghiringhelli, C. Mautalen, G. R. Valzacchi, H. Scaglia and C. Zylberstein: Calcif. Tissue Int. 62 (1998) 465-469.
  9. NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis, and Therapy: JAMA 285 (2001) 785-795.
  10. J. C. Elliot: Structure and chemistry of the apatites and other calcium orthophosphates, (Elsevier, Amsterdam, 1994) pp. 1-389.
  11. T. Nakano, Y. Tabata and Y. Umakoshi: Encyclopedia of Materials, Science and Technology Updates, (Texture and Bone Reinforcement, Elsevier, Oxford, 2005) MS2061, pp. 1-8.
  12. G. Daculsi, J. M. Bouler and R. Z. LeGeros: Int. Rev. Cytol. 172 (1997) 129-191.
  13. N. Sasaki, N. Matsushima, T. Ikawa, H. Yamamura and A. Fukuda: J. Biomech. 22 (1989) 157-164.
  14. G. E. Bacon, P. J. Bacon and R. K. Griffiths: J. Biomech. 13 (1980) 725-729.
  15. K. Sasaki, T. Nakano, J. D. Ferrara, J.-W. Lee and T. Sasaki: Mater. Trans. 49 (2008) 2129-2135.
  16. T. Nakano, W. Fujitani, T. Ishimoto and Y. Umakoshi: J. Phys.: Conf. Ser. 165 (2009) 012084.
  17. R. Tanaka, Y. Shibata, A. Manabe and T. Miyazaki: PLoS One 4 (2009) e5986.
  18. S. Miyabe, T. Nakano, T. Ishimoto, N. Takano, T. Adachi, H. Iwaki, A. Kobayashi, K. Takaoka and Y. Umakoshi: Mater. Trans. 48 (2007) 343-347.
  19. J.-W. Lee, T. Nakano, S. Toyosawa, Y. Tabata and Y. Umakoshi: Mater. Trans. 48 (2007) 337-342.
  20. A. Takimoto, T. Yamane, S. Tanaka, T. Fujii and K. Iwatani: Mem. Fac. Eng. Yamaguchi Univ. 41 (1991) 267-280.
  21. E. Klemetti, P. Vainio and H. Kröger: Gerodontology 11 (1994) 76-79.
  22. S. Picard, N. Lapointe, J. Brown and P. Guertin: Anat. Rec. 291 (2008) 303-307.
  23. C. Tsai, R. Huang, C. Lee, W. Hsiao and L. Yang: J. Oral Maxillofac. Surg. 68 (2010) 1081-1087.
  24. T. Nakano and H. Inui: J. Japan Inst. Metals 76 (2006) 798-804.
  25. T. Ishimoto, T. Nakano, Y. Umakoshi, M. Yamamoto and Y. Tabata: Mater. Sci. Forum 512 (2006) 261-264.
  26. N. Nagisa, T. Nakano, N. Hashiguchi, W. Fujitani, Y. Umakoshi and M. Shimahara: Oral Sci. Int. 7 (2010) 19-25.


[JIM HOME] [JOURNAL ARCHIVES]

© 2012 The Japan Institute of Metals
Comments to us : editjt@jim.or.jp